FastQCFastQC Report
Fri 14 Jun 2019
HKFJGAFXY_n01_PY79_T2_rep3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKFJGAFXY_n01_PY79_T2_rep3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19075011
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCGAGCTTGAGCTGCGAGCCGAGAGGCGATCTCGTAAACACGCACTTAAA208850.10948879662507141No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTCACA54400.022.58277128
CACACGT58350.021.29385212
ACTCCAG58300.020.89214923
ACACGTC63750.019.70974513
CAGTCAC66000.018.9318627
CTCCAGT65050.018.72414824
GAACTCC67300.018.20226521
GGTGGTG37100.017.9244757
AGGTGGT38100.017.4541076
GTCACAT70800.017.45060329
AGCACAC77900.016.57889710
TCCAGTC75950.016.35952825
ACGTCTG74500.016.34894415
GCACACG75750.016.31018311
ACTCTAG28650.016.0035275
CCAGTCA76200.015.93835626
GTGATAG53150.015.9360663
GTGGTGA43100.015.7540158
CTCTAGT27900.015.4301426
TGGGAGT90350.015.3021221
Produced by FastQC (version 0.11.5)