FastQCFastQC Report
Fri 14 Jun 2019
HKFJGAFXY_n01_PY79_T2_rep2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKFJGAFXY_n01_PY79_T2_rep2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4661754
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGCGATAGATCTCGTAT76870.16489501591032046TruSeq Adapter, Index 1 (97% over 36bp)
GCGAGGTTTCTTATAAGCTTTTTTCATTGAGCATCCCTCCTAAAATAGAG50230.10774914334819041No Hit
TCGAGCTTGAGCTGCGAGCCGAGAGGCGATCTCGTAAACACGCACTTAAA48590.1042311541964677No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGATCT15200.037.0722939
AGATCTC16350.034.8928940
GCGATAG17250.033.88398435
GATAGAT17250.033.47818837
AGTCACA18450.032.05955528
ACTCCAG18550.031.88672423
ATAGATC18550.030.56597338
CGATAGA19650.029.923636
GAACTCC20400.029.16660521
CACACGT20700.029.08206212
ACACGTC21000.028.83327113
CAGTCAC21550.027.7725627
TCGTATG21650.027.32095745
GTATGCC21700.027.25800747
TCCAGTC21900.026.84925825
GTCACAG22050.026.82533829
CTCCAGT22800.026.24994324
GCACACG22850.026.0393311
CACGTCT23100.025.7575214
ACGTCTG23150.025.55070115
Produced by FastQC (version 0.11.5)