FastQCFastQC Report
Fri 14 Jun 2019
HKFJGAFXY_n01_PY79_T2_rep1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKFJGAFXY_n01_PY79_T2_rep1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3784012
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGGCTATGATCTCGTAT100920.26670105697339225TruSeq Adapter, Index 7 (97% over 36bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATGCC20650.038.13551347
CACACGT21100.037.48807512
ACTCCAG22000.037.22719623
AGTCACC21100.037.15632228
TCGTATG22200.035.78821645
GCACACG22350.035.54802711
CTATGAT21950.034.7607537
TCCAGTC23600.034.40670825
CGTATGC23550.033.7366646
CCGGCTA23850.033.605833
ACGTCTG24300.032.8394415
ACACGTC24500.032.42850513
CTCGTAT23450.032.38799344
GAACTCC25600.032.12883821
GGCTATG24800.031.89509635
CAGTCAC26050.031.84254527
CACGTCT25100.031.51387814
GCTATGA26000.030.82685936
CTCCAGT27050.029.88903224
CGGCTAT26850.029.7206134
Produced by FastQC (version 0.11.5)