FastQCFastQC Report
Fri 14 Jun 2019
HKFJGAFXY_n01_PY79_T1_rep3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKFJGAFXY_n01_PY79_T1_rep3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2888306
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCGAGCTTGAGCTGCGAGCCGAGAGGCGATCTCGTAAACACGCACTTAAA29080.10068185296156294No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGCGC10800.036.6203635
ACTCTCG10800.036.29628832
TCTCGCG11500.034.0869534
GTCACTC11600.033.79309529
TCACTCT12100.032.39668730
AGTCACT12150.032.26336728
CACACGT12300.031.86991112
CGCGCAT12600.031.11110337
ACTCCAG12600.031.11110323
CTCTCGC12950.030.27026233
CACTCTC13400.029.77611231
GCACACG13550.029.18818511
CTCCAGT13800.028.91303824
CCAGTCA13550.028.6715826
CAGTCAC13650.028.46153327
TCCAGTC14450.027.85466425
AGCACAC14650.027.47439810
TCGCGCA14500.027.27585636
ACGTCTG15500.025.96773515
ACACGTC17100.023.74268313
Produced by FastQC (version 0.11.5)