FastQCFastQC Report
Fri 14 Jun 2019
HKFJGAFXY_n01_PY79_T1_rep2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKFJGAFXY_n01_PY79_T1_rep2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3726352
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACTAAT8450.038.93490631
ACTCCAG11800.027.88135123
CACACGT12050.027.01244412
GCACACG12400.025.9677411
ACTAATG13350.024.90636332
TGCGCAT13850.024.5126337
GTCACTA13700.023.7591229
CAGTCAC14350.023.17072727
AGTCACT14450.023.01037828
ATGCGCA14000.022.99999836
AGCACAC15200.022.10525910
CTCCAGT15400.022.0454524
ACGTCTG15700.020.7324815
GAACTCC16900.019.46745321
TAATGCG16550.019.4561934
TCCAGTC17950.019.30361725
ACACGTC17250.019.07245813
CCAGTCA17450.018.65329226
AATGCGC17850.018.62744735
CTCTAGT7200.018.472226
Produced by FastQC (version 0.11.5)