FastQCFastQC Report
Fri 14 Jun 2019
HKFJGAFXY_n01_PY79_T1_rep1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKFJGAFXY_n01_PY79_T1_rep1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5871557
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCGAGCTTGAGCTGCGAGCCGAGAGGCGATCTCGTAAACACGCACTTAAA61260.10433348428704686No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCCGCG25600.039.37523333
ACTCCGC26400.037.91688532
ACTCCAG26750.037.81298423
CACACGT29400.035.11895412
GCACACG29550.034.94068511
AGTCACT30650.033.57279228
GTCACTC31650.032.29086729
TCACTCC31800.032.13855430
TCCAGTC32450.031.7102425
ACACGTC33600.030.93741613
GAACTCC34350.030.05814421
ACGTCTG33950.029.99992215
CAGTCAC36150.029.14238427
CCGCGAA35700.028.92173835
CACGTCT36100.028.5040814
CGCGAAA36700.027.46586836
AGCACAC39800.026.2059610
CTCCAGT40300.025.6202824
CGTCTGA41400.025.1931716
CACTCCG41400.025.02430231
Produced by FastQC (version 0.11.5)