FastQCFastQC Report
Fri 14 Jun 2019
HKFJGAFXY_n01_PY79_T0_rep3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKFJGAFXY_n01_PY79_T0_rep3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4688534
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGCGATAGATCTCGTAT109720.23401771214627004TruSeq Adapter, Index 1 (97% over 36bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGATCT21950.034.7606539
AGTCACA23200.034.39638528
GATAGAT24300.032.83934437
ATAGATC23750.032.42089538
CACACGT25250.031.7424212
GTCACAG25500.031.1567129
GCACACG26300.031.00745611
GTATGCC25850.031.00565147
ACTCCAG26500.030.24513623
CAGTCAC26650.030.20623227
CCAGTCA26450.030.16998526
AGATCTC25750.029.76684440
CGATAGA28700.028.17059536
TCGTATG29200.027.80808345
GCGATAG28600.027.77958735
AGCGATA29300.027.35481534
CTCCAGT30700.026.56338924
AGAGCAC31050.026.2639648
CGTATGC31300.025.71872546
GAACTCC32000.025.37487821
Produced by FastQC (version 0.11.5)