FastQCFastQC Report
Fri 14 Jun 2019
HKFJGAFXY_n01_PY79_T0_rep2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKFJGAFXY_n01_PY79_T0_rep2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4582174
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACTAAT13850.040.43351431
ACTCCAG17800.030.87101723
GTCACTA18200.030.38484229
GCACACG19100.029.31927111
CACACGT18950.028.99726312
AGTCACT19000.028.92126728
GAACTCC18900.028.88910521
AGCACAC21100.026.54019210
ACTAATG21200.026.08481632
CAGTCAC22100.025.81467227
CTCCAGT22300.024.6414424
ACACGTC22650.024.26040313
TCCAGTC22900.024.14865325
ATGCGCA22600.023.6946136
TGCGCAT22450.023.3852237
TAATGCG23900.022.99155234
GAGCACA25150.022.9621499
CACGTCT25550.022.19170414
CCAGTCA25300.021.7195326
ACGTCTG26000.021.13454215
Produced by FastQC (version 0.11.5)