FastQCFastQC Report
Fri 14 Jun 2019
HKFJGAFXY_n01_PY79_T0_rep1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKFJGAFXY_n01_PY79_T0_rep1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4814931
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTGAAGCTATCTCGTAT50140.10413441023350074TruSeq Adapter, Index 19 (97% over 38bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACACGT13300.030.26306312
GTCACCT14950.026.92299329
GCACACG15100.026.88733311
CTATCTC14300.026.43348340
AGTCACC15100.026.4237628
GTATGCC15600.025.35248647
ACTCCAG16550.024.32016623
TATCTCG16050.023.55132941
CCAGTCA17900.022.87702426
GAAGCTA18450.022.38475236
GAACTCC18400.022.25536521
AGCTATC18300.021.80321138
TGAACTC19150.021.38374520
TCGTATG19050.020.7610945
CTCCAGT19200.020.59889424
CAGTCAC20600.020.21838427
AGGGAGG17150.019.79585614
ACACGTC20450.019.51094213
TAGGGTT10250.019.46335422
AGCACAC21100.019.40752210
Produced by FastQC (version 0.11.5)