FastQCFastQC Report
Fri 7 Apr 2017
HKFFYBGX2_n01_nlp7ntest18.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKFFYBGX2_n01_nlp7ntest18.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14845798
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGATAACATGGCCATCATCAAGGAGTTCATGCGCTTCAAGGTGCACATGGAGGGCTCCGTGAACGGCCACGAGTT150020.10105216304303749No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCCTA29400.018.8676138
ACTCTAG46650.017.8025135
ACTACCC32750.017.466426
CTAGTTG42200.016.2472178
CTCTAGT48200.015.6568456
GGGGCCC3351.9463187E-1015.4579469
TACTCTA46700.015.2745324
CTACCCT38550.015.0149797
CCTACAC47900.014.52966911
TCTAGTT53500.014.2324077
GGGCGCC67700.014.12759166
AGGGCGC69300.013.80476765
GTTACCT48350.013.68193514
CTAATGC48000.013.64209221
ACCTCTA48550.013.55460617
GACACCT49200.013.32322730
GGGCAGG61750.013.25109568
GGACACC46400.013.23372129
GGATAAC97650.013.0246231
CGCGTAT13000.012.9916331
Produced by FastQC (version 0.11.2)