FastQCFastQC Report
Fri 7 Apr 2017
HKFFYBGX2_n01_nlp7ntest17.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKFFYBGX2_n01_nlp7ntest17.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20183377
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGATAACATGGCCATCATCAAGGAGTTCATGCGCTTCAAGGTGCACATGGAGGGCTCCGTGAACGGCCACGAGTT208480.103292922685832No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGATAAC133250.014.5899121
GGGCGCC83650.014.07410266
AGGGCGC88100.013.56214565
CAGGGCG93950.012.60865464
GGGCAGG78700.012.062592568
TACCCTA26700.011.8735238
GCAAGGG65700.011.8047811
GCGCCGT104750.011.69922368
TGGCCGC104200.011.57774320
TAACATG152750.011.3259334
CAAGGGC65850.011.3049252
CGGCAAC88800.011.2182641
GGCAGGG87350.011.0256669
CGCCCTT108750.010.88928257
AGTGGCC111400.010.82687518
TAGTATG89000.010.78147634
CGAAGGG89900.010.71805264
AGCGTCG116350.010.66203910
CATGGCC160150.010.6508047
GCGTCGT114200.010.62134711
Produced by FastQC (version 0.11.2)