FastQCFastQC Report
Fri 7 Apr 2017
HKFFYBGX2_n01_nlp7ntest16.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKFFYBGX2_n01_nlp7ntest16.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26243303
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGATAACATGGCCATCATCAAGGAGTTCATGCGCTTCAAGGTGCACATGGAGGGCTCCGTGAACGGCCACGAGTT320250.12203113304754361No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGATAAC180750.014.8182781
TACCCTA40500.014.1235628
CGGGCAT25750.012.9852271
GGGCGCC118500.012.93643866
GGGGCAC26400.012.92372819
AGGGCGC123100.012.56797865
CTAGTTG60700.012.5456968
GGGGGCA26800.012.47253118
GGGGCCC6100.011.8843869
CAGGGCG131500.011.63473764
ACTACCC51100.011.6000086
TGGCCGC138000.011.51356520
TAACATG219950.011.5009474
GCAAGGG79050.011.3813481
GGGCAGG103250.011.36839568
ACTCTAG74500.011.1486495
GATAACA248800.010.9853762
CGGCAAC123950.010.9851411
GCGCCGT143200.010.80053568
TACTCTA71700.010.7668924
Produced by FastQC (version 0.11.2)