FastQCFastQC Report
Fri 7 Apr 2017
HKFFYBGX2_n01_nlp7ntest15.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKFFYBGX2_n01_nlp7ntest15.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14471398
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGATAACATGGCCATCATCAAGGAGTTCATGCGCTTCAAGGTGCACATGGAGGGCTCCGTGAACGGCCACGAGTT158560.10956785239408108No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGATAAC97250.014.8518381
GGGCGCC59300.013.5659366
AGGGCGC61400.013.21734165
TACCCTA21300.012.9414988
GCCGAAT23600.012.7075841
CGGGCAT14750.012.6199471
CAGGGCG64500.012.36916764
GCAAGGG45050.011.8602191
TAACATG116200.011.8036734
CGGCAAC68600.011.7081311
TGGCCGC73250.011.292232520
CAAGGGC46350.011.2277942
GATAACA133500.011.1008032
CATGGCC119100.011.0516537
CGCCCTT74900.011.016952557
GCGCCGT73750.011.00102468
GGCCATC117150.011.00031110
AGTGGCC77650.010.96097518
GGGGCAC17350.010.92443419
ATAACAT129100.010.6776073
Produced by FastQC (version 0.11.2)