FastQCFastQC Report
Fri 7 Apr 2017
HKFFYBGX2_n01_nlp7ntest14.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKFFYBGX2_n01_nlp7ntest14.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27493912
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGATAACATGGCCATCATCAAGGAGTTCATGCGCTTCAAGGTGCACATGGAGGGCTCCGTGAACGGCCACGAGTT288920.10508508210835912No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGCGCC99400.016.18541166
AGGGCGC101850.015.86791265
GGATAAC163850.015.0428411
CAGGGCG109250.014.57319664
TGGCCGC121800.013.55469420
GCGCCGT123150.013.23144368
AGCGTCG137750.012.50796610
AGTGGCC132650.012.49558118
GCGTCGT136700.012.200623511
CGCCCTT131750.012.15924557
TACCCTA33950.011.5710258
TAACATG202450.011.3206724
CAGCGTC151850.011.3010939
TCAGCGT151650.011.2251278
CGTCGTA150300.011.21123612
TCAGGGC145150.011.206676563
GTCGTAG150650.011.02511413
CATGGCC202350.011.0181887
GGCCATC202050.010.86397810
CTCAGCG157650.010.7979697
Produced by FastQC (version 0.11.2)