FastQCFastQC Report
Fri 7 Apr 2017
HKFFYBGX2_n01_nlp7ntest13.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKFFYBGX2_n01_nlp7ntest13.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22082096
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGATAACATGGCCATCATCAAGGAGTTCATGCGCTTCAAGGTGCACATGGAGGGCTCCGTGAACGGCCACGAGTT326050.14765355607547398No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCCTA45550.018.836158
CTAGTTG57250.017.0330378
ACTACCC51500.016.862916
ACTCTAG69800.016.1451975
CTACCCT55950.015.3965027
CTCTAGT68450.015.3555586
TACTCTA66200.015.09701254
TCTAGTT75800.014.8648567
GGGGCCC4700.014.68978769
GGATAAC184300.014.2707431
GTTACCT67500.013.78282414
ACCTCTA68000.013.73224517
CCTACAC74250.013.68994911
CTAATGC69500.013.19113721
GGGCGCC116150.012.96037666
AGGGCGC121350.012.77801465
TAGTTGT85550.012.5261789
CAGGGCG125600.012.18182364
GGACACC73650.012.08382329
GCAAGGG80650.012.0101751
Produced by FastQC (version 0.11.2)