FastQCFastQC Report
Fri 7 Apr 2017
HKFFYBGX2_n01_nlp7ntest12.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKFFYBGX2_n01_nlp7ntest12.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16874968
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGATAACATGGCCATCATCAAGGAGTTCATGCGCTTCAAGGTGCACATGGAGGGCTCCGTGAACGGCCACGAGTT174030.10312908445218978No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGATAAC106050.014.691561
GGGGCCC3851.0186341E-1014.34744369
GGGCGCC62500.012.59564366
AGGGCGC66050.012.07781865
CGCGTAT17150.011.8584521
TAACATG125500.011.5878624
CAGGGCG70950.011.19624864
TGGCCGC78600.010.91794720
CATGGCC129550.010.87801557
GATAACA147900.010.812072
TACCCTA20150.010.7727548
GGCCATC128600.010.74396610
ACATGGC142300.010.5828376
CGGCAAC74200.010.498921
CGCGGAT21050.010.4801621
GCGCCGT77700.010.21989768
ATAACAT145250.010.130863
ATGGCCA140650.010.0439688
GGAAAAT131750.09.9681481
TGGCCAT140050.09.9639279
Produced by FastQC (version 0.11.2)