FastQCFastQC Report
Fri 7 Apr 2017
HKFFYBGX2_n01_nlp7ntest11.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKFFYBGX2_n01_nlp7ntest11.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14640995
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCCTA20950.015.2963278
GGGCGCC55250.014.06012566
GGATAAC93050.013.5574361
AGGGCGC57400.013.41584765
ACTCTAG43800.013.3754245
CTAGTTG34800.013.3672718
CTCTAGT41450.012.7201066
CAGGGCG62750.012.27311964
ACTACCC26900.011.9139156
TACTCTA42200.011.7592554
TGGCCGC68700.011.63932520
GCGCCGT69100.011.49122868
AGCGTCG72550.011.25631410
CGGCAAC64050.011.2470871
TCTAGTT50850.010.9777237
CGCGTAT15550.010.86121751
AGTGGCC73650.010.854897518
TAACATG109950.010.8131854
CCTACAC39900.010.79495711
GCCGAAT24700.010.7450061
Produced by FastQC (version 0.11.2)