FastQCFastQC Report
Fri 7 Apr 2017
HKFFYBGX2_n01_nlp7ntest10.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKFFYBGX2_n01_nlp7ntest10.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18739365
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGATAAC112500.014.401281
GGGCGCC69700.013.91944766
AGGGCGC71000.013.81349765
CAGGGCG77550.012.73673364
GGGGCCC3852.2653694E-812.55356169
TGGCCGC84400.012.00608920
GCGCCGT86150.011.180531568
GCAAGGG57050.011.1782011
AGTGGCC91350.011.165718
CGCCCTT88900.011.14597557
CGCGTAT17400.011.0941641
CGCGGGG8250.010.8636351
TAACATG138000.010.8633834
TAGTATG80950.010.83025634
GCGTCGT94300.010.81599811
CGGGCAT17550.010.8029241
AGCGTCG96450.010.68210110
CAAGGGC56600.010.5952712
CATGGCC140250.010.4171657
GGCCATC139750.010.35583710
Produced by FastQC (version 0.11.2)