FastQCFastQC Report
Fri 7 Apr 2017
HKFFYBGX2_n01_nlp7ntest09.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKFFYBGX2_n01_nlp7ntest09.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17161949
Sequences flagged as poor quality0
Sequence length75
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGATTCTAGCATCACTGGTGGACAGCAAATCGGTCGGGATCTGTAGGACGATCACGATAAGGATCCGATGGTG483340.28163467913813284No Hit
GCTGACTCTAGCAGATCTATCGATTCTAGCATCACTGGTGGACAGCAAATCGGTCGGGATCTGTAGGACGATCAC462750.26963720728921875No Hit
AGCAGATCTATCGATTCTAGCATCACTGGTGGACAGCAAATCGGTCGGGATCTGTAGGACGATCACGATAAGGAT354510.20656744755505335No Hit
CGGGAATTGGATCCGGTACCGAGCTCGAATTCTTACAGCTCGTCCTTCTTGTACAGCTCGTCCATGCCGAGAGTG292800.17060999307246513No Hit
CCGGTACCGAGCTCGAATTCTTACAGCTCGTCCTTCTTGTACAGCTCGTCCATGCCGAGAGTGATCCCGGCGGCG259940.15146298360401841No Hit
CGGCATCAAGGTGAACTTCAAGATCCGCCACAACATCGAGGACGGCAGCGTGCAGCTCGCCGACCACTACCAGCA224140.13060288199201617No Hit
CTTTGCTCAGGGCGGACTGGGTGCTCAGGTAGTGGTTGTCGGGCAGCAGCACGGGGCCGTCGCCGATGGGGGTGT210630.1227308157132969No Hit
CTGGAGTACAACTACAACAGCCACAACGTCTATATCATGGCCGACAAGCAGAAGAACGGCATCAAGGTGAACTTC202690.11810430155689193No Hit
GCAGATCTATCGATTCTAGCATCACTGGTGGACAGCAAATCGGTCGGGATCTGTAGGACGATCACGATAAGGATC202560.11802855258455786No Hit
CGGCAACATCCTGGGGCACAAGCTGGAGTACAACTACAACAGCCACAACGTCTATATCATGGCCGACAAGCAGAA182480.10632824978095436No Hit
GCAAATCGGTCGGGATCTGTAGGACGATCACGATAAGGATCCGATGGTGAGCAAGGGCGAGGAGCTGTTCACCGG179890.10481909717829833No Hit
CTCAGGTAGTGGTTGTCGGGCAGCAGCACGGGGCCGTCGCCGATGGGGGTGTTCTGCTGGTAGTGGTCGGCGAGC178890.10423641277572844No Hit
CGGATCTTGAAGTTCACCTTGATGCCGTTCTTCTGCTTGTCGGCCATGATATAGACGTTGTGGCTGTTGTAGTTG173680.10120062703833929No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTGACT92600.037.0359151
ACTCTAG110450.033.260715
GACTCTA112800.031.2538934
CTGACTC110550.030.8624532
CTAGCAG128650.026.8368938
TGACTCT147100.024.2474043
CTCTAGC146950.024.1309536
TAGCAGA152250.022.563739
TCTAGCA394050.018.2405227
TCTATCG210600.016.05050716
CTATCGA213000.015.85361817
GATCTAT226150.014.94670514
AGATCTA229400.014.84000513
AGCAGAT234100.014.79236710
GCAGATC234050.014.63354511
ATCTATC235500.014.38254315
TATCGAT238250.014.13054118
CGGGAAT177350.014.1290891
CCCGGCG141850.013.19211466
ATTCTAG289600.013.0779365
Produced by FastQC (version 0.11.2)