FastQCFastQC Report
Fri 7 Apr 2017
HKFFYBGX2_n01_nlp7ntest05.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKFFYBGX2_n01_nlp7ntest05.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18814361
Sequences flagged as poor quality0
Sequence length75
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGACTCTAGCAGATCTATCGATTCTAGCATCACTGGTGGACAGCAAATCGGTCGGGATCTGTAGGACGATCAC501040.2663072107524672No Hit
ATCGATTCTAGCATCACTGGTGGACAGCAAATCGGTCGGGATCTGTAGGACGATCACGATAAGGATCCGATGGTG487470.25909463520977405No Hit
AGCAGATCTATCGATTCTAGCATCACTGGTGGACAGCAAATCGGTCGGGATCTGTAGGACGATCACGATAAGGAT363950.1934426579781264No Hit
CGGGAATTGGATCCGGTACCGAGCTCGAATTCTTACAGCTCGTCCTTCTTGTACAGCTCGTCCATGCCGAGAGTG325720.17312307337995694No Hit
CCGGTACCGAGCTCGAATTCTTACAGCTCGTCCTTCTTGTACAGCTCGTCCATGCCGAGAGTGATCCCGGCGGCG268650.1427898614255355No Hit
CTGGAGTACAACTACAACAGCCACAACGTCTATATCATGGCCGACAAGCAGAAGAACGGCATCAAGGTGAACTTC244750.1300867991211607No Hit
CGGCATCAAGGTGAACTTCAAGATCCGCCACAACATCGAGGACGGCAGCGTGCAGCTCGCCGACCACTACCAGCA241490.12835408016248864No Hit
CTTTGCTCAGGGCGGACTGGGTGCTCAGGTAGTGGTTGTCGGGCAGCAGCACGGGGCCGTCGCCGATGGGGGTGT220370.11712861255293229No Hit
GCAGATCTATCGATTCTAGCATCACTGGTGGACAGCAAATCGGTCGGGATCTGTAGGACGATCACGATAAGGATC208340.11073456069010262No Hit
CGGCAACATCCTGGGGCACAAGCTGGAGTACAACTACAACAGCCACAACGTCTATATCATGGCCGACAAGCAGAA206100.10954398079211938No Hit
CTCAGGTAGTGGTTGTCGGGCAGCAGCACGGGGCCGTCGCCGATGGGGGTGTTCTGCTGGTAGTGGTCGGCGAGC201390.1070405739530564No Hit
GCAAATCGGTCGGGATCTGTAGGACGATCACGATAAGGATCCGATGGTGAGCAAGGGCGAGGAGCTGTTCACCGG199980.10629114642798658No Hit
CGGATCTTGAAGTTCACCTTGATGCCGTTCTTCTGCTTGTCGGCCATGATATAGACGTTGTGGCTGTTGTAGTTG194470.103362532482501No Hit
GCGGACTTGAAGAAGTCGTGCTGCTTCATGTGGTCGGGGTAGCGGCTGAAGCACTGCACGCCGTAGGTCAGGGTG191120.1015819777243564No Hit
GGCGATGCCACCTACGGCAAGCTGACCCTGAAGTTCATCTGCACCACCGGCAAGCTGCCCGTGCCCTGGCCCACC189140.10052959013596051No Hit
CGGCAACAGGATTCAATCTTAAGAAACTTTATTGCCAAATGTTTGAACGATCGGGAATTGGATCCGGTACCGAGC188690.1002904111385978No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTGACT99500.038.0044631
CTGACTC117550.032.103682
ACTCTAG126200.031.94975
GACTCTA128200.029.9996664
CTAGCAG133050.028.281168
CTCTAGC158800.024.6959136
TGACTCT157550.024.5644053
TAGCAGA161050.023.385569
TCTAGCA412800.018.1723757
TCTATCG223500.016.57391216
CTATCGA224600.016.49282817
GATCTAT234350.015.86529914
AGCAGAT242400.015.7221110
GCAGATC244100.015.30205711
AGATCTA245950.015.2431213
ATCTATC249550.014.95418315
CGGGAAT196350.014.7117451
TATCGAT253500.014.68101418
CCCGGCG151250.013.37691266
ATTCTAG299000.012.6322185
Produced by FastQC (version 0.11.2)