FastQCFastQC Report
Fri 7 Apr 2017
HKFFYBGX2_n01_nlp7ntest01.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKFFYBGX2_n01_nlp7ntest01.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22293685
Sequences flagged as poor quality0
Sequence length75
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGACTCTAGCAGATCTATCGATTCTAGCATCACTGGTGGACAGCAAATCGGTCGGGATCTGTAGGACGATCAC600450.26933636139561495No Hit
ATCGATTCTAGCATCACTGGTGGACAGCAAATCGGTCGGGATCTGTAGGACGATCACGATAAGGATCCGATGGTG534540.23977193541579153No Hit
AGCAGATCTATCGATTCTAGCATCACTGGTGGACAGCAAATCGGTCGGGATCTGTAGGACGATCACGATAAGGAT412490.18502549040232694No Hit
CGGGAATTGGATCCGGTACCGAGCTCGAATTCTTACAGCTCGTCCTTCTTGTACAGCTCGTCCATGCCGAGAGTG357970.16057013454707017No Hit
CCGGTACCGAGCTCGAATTCTTACAGCTCGTCCTTCTTGTACAGCTCGTCCATGCCGAGAGTGATCCCGGCGGCG321340.14413947268026797No Hit
CGGCATCAAGGTGAACTTCAAGATCCGCCACAACATCGAGGACGGCAGCGTGCAGCTCGCCGACCACTACCAGCA289350.12979011769476423No Hit
CTGGAGTACAACTACAACAGCCACAACGTCTATATCATGGCCGACAAGCAGAAGAACGGCATCAAGGTGAACTTC276060.12382878828690726No Hit
CTTTGCTCAGGGCGGACTGGGTGCTCAGGTAGTGGTTGTCGGGCAGCAGCACGGGGCCGTCGCCGATGGGGGTGT253380.11365550378952605No Hit
CGGCAACATCCTGGGGCACAAGCTGGAGTACAACTACAACAGCCACAACGTCTATATCATGGCCGACAAGCAGAA244460.10965437073323679No Hit
GGCGATGCCACCTACGGCAAGCTGACCCTGAAGTTCATCTGCACCACCGGCAAGCTGCCCGTGCCCTGGCCCACC244250.10956017365455734No Hit
GCAGATCTATCGATTCTAGCATCACTGGTGGACAGCAAATCGGTCGGGATCTGTAGGACGATCACGATAAGGATC242520.10878416914924564No Hit
GCGGACTTGAAGAAGTCGTGCTGCTTCATGTGGTCGGGGTAGCGGCTGAAGCACTGCACGCCGTAGGTCAGGGTG241960.10853297693943374No Hit
GCAAATCGGTCGGGATCTGTAGGACGATCACGATAAGGATCCGATGGTGAGCAAGGGCGAGGAGCTGTTCACCGG234600.10523159361047758No Hit
CGGATCTTGAAGTTCACCTTGATGCCGTTCTTCTGCTTGTCGGCCATGATATAGACGTTGTGGCTGTTGTAGTTG227510.10205132081125216No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTGACT117050.039.2860371
ACTCTAG141700.034.073135
CTGACTC144900.031.538522
GACTCTA151700.030.9637834
CTAGCAG158500.028.6968258
CTCTAGC185250.025.5044046
TGACTCT188200.024.5741563
TAGCAGA191600.023.77529
TCTAGCA470700.018.4478827
CTATCGA256450.017.40063317
TCTATCG257400.017.3629916
GATCTAT273500.016.30298414
AGCAGAT286600.016.06269810
AGATCTA280200.015.93771513
ATCTATC284200.015.786180515
TATCGAT285150.015.69828618
GCAGATC287500.015.68880911
CGGGAAT230850.013.7525611
CCCGGCG176600.013.11834366
CAGATCT348100.013.0268412
Produced by FastQC (version 0.11.2)