FastQCFastQC Report
Fri 7 Apr 2017
HKFFYBGX2_n01_damid04.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKFFYBGX2_n01_damid04.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2470387
Sequences flagged as poor quality0
Sequence length75
%GC52

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGG120156048.63853315290277TruSeq Adapter, Index 4 (100% over 63bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATATCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGG192500.7792301368166202TruSeq Adapter, Index 4 (98% over 63bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAG172920.6999713000432726TruSeq Adapter, Index 4 (100% over 63bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAATGG158720.6424904276131634TruSeq Adapter, Index 4 (100% over 63bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAATG119410.4833655617520655TruSeq Adapter, Index 4 (100% over 63bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAGGG63000.2550207720490757TruSeq Adapter, Index 4 (100% over 63bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAACG57340.23210938205228573TruSeq Adapter, Index 4 (100% over 63bp)
GGTCGCGGCCGAGGATCCATTGGAGGGCAAGTCTGGTGCCAGCAGCCGCGGTAATTCCAGCTCCAATAGCGTATA35740.1446736887783169No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG27970.11322112689226425No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGT27030.10941605505534154TruSeq Adapter, Index 4 (100% over 63bp)
GGTCGCGGCCGAGGATCGAAATCCTATGATGTTATCCCATGCTAATGTATCCAGAGCGTAGGCTTGCTTTGAGCA27000.10929461659246102No Hit
GGTCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGG25640.10378940627521113TruSeq Adapter, Index 4 (98% over 63bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTGAAA1401100.067.9769260
AAAAAGG1311250.067.94626669
TTGAAAA1403950.067.9138161
GCTTGAA1401900.067.87051459
CTCGTAT1368950.067.87034642
TGCTTGA1400250.067.8660558
CCAATCT1360750.067.856637
GTCTTCT1400950.067.8468252
AATCTCG1365750.067.81818439
CAATCTC1362700.067.8168238
TCTCGTA1368250.067.8016241
TCGTATG1408000.067.7746643
TCTTCTG1402400.067.7597253
ATCTCGT1367800.067.7594540
TGAAAAA1411650.067.7586762
TTCTGCT1399800.067.7584155
GAAAAAA1415400.067.7572763
TCTGCTT1399800.067.7545756
CCGTCTT1404150.067.75250
ACCAATC1361000.067.748736
Produced by FastQC (version 0.11.2)