FastQCFastQC Report
Fri 7 Apr 2017
HKFFYBGX2_n01_damid03.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKFFYBGX2_n01_damid03.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8457886
Sequences flagged as poor quality0
Sequence length75
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGG557957965.9689548901463TruSeq Adapter, Index 3 (100% over 63bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAG1056271.2488581662131648TruSeq Adapter, Index 3 (100% over 63bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAATG821070.970774493768301TruSeq Adapter, Index 3 (100% over 63bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAATGG687110.8123897626428165TruSeq Adapter, Index 3 (100% over 63bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAACG218980.2589063035373142TruSeq Adapter, Index 3 (100% over 63bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTATGCCGTCTTCTGCGTGAAAAAAAAAAGG151160.17872078200155453TruSeq Adapter, Index 3 (98% over 63bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCGCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGG148750.17587137022182614TruSeq Adapter, Index 3 (98% over 63bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGT144390.17071641779045021TruSeq Adapter, Index 3 (100% over 63bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAGGG141760.1676068937320744TruSeq Adapter, Index 3 (100% over 63bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATATCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGG140440.16604622006019TruSeq Adapter, Index 3 (98% over 63bp)
GGTCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGG134660.15921236110299902TruSeq Adapter, Index 3 (98% over 63bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG126900.1500374916379814No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTATGCCGTCTTATGCTTGAAAAAAAAAAGG111040.131285761004582TruSeq Adapter, Index 3 (98% over 63bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGG103390.12224094767888807TruSeq Adapter, Index 3 (98% over 63bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTATGCCGTCGTCTGCTTGAAAAAAAAAAGG95600.11303060835769127TruSeq Adapter, Index 3 (98% over 63bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAAAAGG6099000.068.3972369
CTTGAAA6511850.068.1214460
GCTTGAA6496200.068.09772559
TGCTTGA6458350.068.09041658
TTGAAAA6541250.068.0788861
GTCTTCT6446700.068.0430452
TCTGCTT6449800.068.0349456
CTGCTTG6432550.068.0342557
TGAAAAA6621950.068.0260162
TTCTGCT6415800.068.024255
TCTTCTG6448550.068.0154853
CCGTCTT6514650.068.0144850
GCCGTCT6501400.068.0080849
CTCGTAT6487050.068.0068342
TCGTATG6526550.067.9954143
TCTCGTA6482450.067.9939641
CTTCTGC6449350.067.9931354
CGTATGC6555200.067.9911244
TATGCCG6559000.067.983646
GTATGCC6570600.067.9812645
Produced by FastQC (version 0.11.2)