FastQCFastQC Report
Fri 7 Apr 2017
HKFFYBGX2_n01_damid02.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKFFYBGX2_n01_damid02.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5570573
Sequences flagged as poor quality0
Sequence length75
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGG201937436.250741171509645TruSeq Adapter, Index 2 (100% over 63bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAG409940.7359027518354037TruSeq Adapter, Index 2 (100% over 63bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAATG293370.5266424118308835TruSeq Adapter, Index 2 (100% over 63bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAATGG201970.3625659335224581TruSeq Adapter, Index 2 (100% over 63bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCGCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGG127960.22970706963179552TruSeq Adapter, Index 2 (98% over 63bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGCCGTCTTCTGCGTGAAAAAAAAAAGG112350.20168481770187738TruSeq Adapter, Index 2 (98% over 63bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAACG72830.13074058988186674TruSeq Adapter, Index 2 (100% over 63bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGCCGGCTTCTGCTTGAAAAAAAAAAGG66610.11957477264906143TruSeq Adapter, Index 2 (98% over 63bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGCCGTCTTATGCTTGAAAAAAAAAAGG62760.11266345490849863TruSeq Adapter, Index 2 (98% over 63bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGCCGTCGTCTGCTTGAAAAAAAAAAGG60910.10934243209809835TruSeq Adapter, Index 2 (98% over 63bp)
GGTCGCGGCCGAGGATCCATTGGAGGGCAAGTCTGGTGCCAGCAGCCGCGGTAATTCCAGCTCCAATAGCGTATA59560.10691898302023868No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGT59450.10672151679907974TruSeq Adapter, Index 2 (100% over 63bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAAAAGG2285600.068.2188369
CTTGAAA2409500.067.8810560
CTCGTAT2403250.067.870642
TGCTTGA2384850.067.84440658
GCTTGAA2403100.067.8275459
TCTCGTA2409400.067.8154941
TCGTATG2409850.067.79069543
GTCTTCT2373950.067.78168552
GTATCTC2429100.067.755838
TGTATCT2438650.067.7521337
CGTATGC2435950.067.7288744
TTGAAAA2428750.067.6971161
ATCTCGT2423100.067.6966440
GTATGCC2445300.067.695245
GATGTAT2500550.067.6793935
CCGTCTT2413750.067.67300450
TCTGCTT2382400.067.6482856
TATCTCG2429300.067.6460839
CTGCTTG2374450.067.6196157
TCTTCTG2382450.067.6072753
Produced by FastQC (version 0.11.2)