FastQCFastQC Report
Fri 7 Apr 2017
HKFFYBGX2_n01_damid01.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKFFYBGX2_n01_damid01.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2509731
Sequences flagged as poor quality0
Sequence length75
%GC52

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGG123425149.178617150602996TruSeq Adapter, Index 1 (100% over 63bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAATGG192320.766297264527553TruSeq Adapter, Index 1 (100% over 63bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAG153930.6133326639388843TruSeq Adapter, Index 1 (100% over 63bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAATG124690.49682615387864276TruSeq Adapter, Index 1 (100% over 63bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATATCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGG91370.36406292148441405TruSeq Adapter, Index 1 (98% over 63bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAGGG71310.28413403667564374TruSeq Adapter, Index 1 (100% over 63bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAACG60240.2400257238723991TruSeq Adapter, Index 1 (100% over 63bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG30170.12021208647460624No Hit
GGTCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGG28550.11375721143022897TruSeq Adapter, Index 1 (98% over 63bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGCTCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGG28510.11359783179950361TruSeq Adapter, Index 1 (98% over 63bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGG26820.10686404240135695TruSeq Adapter, Index 1 (98% over 63bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAATAGG25680.10232172292568406TruSeq Adapter, Index 1 (100% over 63bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGT25470.1014849798643759TruSeq Adapter, Index 1 (100% over 63bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAAAAGG1338800.067.9560169
CTTGAAA1432200.067.95357560
GCTTGAA1431350.067.9170359
TGCTTGA1429700.067.915158
CTCGTAT1412150.067.8949242
TTGAAAA1434950.067.8917861
GTCTTCT1431000.067.8778752
TCTCGTA1407450.067.8665741
TCGTATG1434400.067.8547743
CCGTCTT1432400.067.8256950
CTGCTTG1430550.067.81428557
TCTGCTT1430550.067.8075256
CGTATGC1436100.067.7984844
TCTTCTG1432200.067.79719553
GTATGCC1436150.067.78995545
TTCTGCT1431100.067.7884155
ATCTCGT1404450.067.7878940
GAAAAAA1443300.067.7526263
ACGATCT1401700.067.73839637
GCCGTCT1435000.067.729149
Produced by FastQC (version 0.11.2)