FastQCFastQC Report
Mon 29 Jul 2024
HKF3VBGXV_n01_HW50.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKF3VBGXV_n01_HW50.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences37925079
Sequences flagged as poor quality0
Sequence length76
%GC36

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATGTCGTATGC2234330.5891431366563534TruSeq Adapter, Index 5 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATGGCGTATGC1054620.2780798426286732TruSeq Adapter, Index 5 (97% over 41bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGC1026730.27072586981295416TruSeq Adapter, Index 5 (100% over 50bp)
CCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCC926920.2444081922677076No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATGTCGTATTC442030.11655348166842315TruSeq Adapter, Index 5 (97% over 48bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)