FastQCFastQC Report
Tue 4 Jun 2019
HKCHVBGXB_3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKCHVBGXB_3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences354491733
Sequences flagged as poor quality0
Sequence length92
%GC86

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG26414137574.51270379837038No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT3464300.069.253721
AGCAGTG3646500.063.195972
GCAGTGG4111150.055.1103443
CAGTGGT4305150.052.3088764
AGTGGTA4365600.051.4336435
GGTATCA4630700.047.7041858
GTGGTAT4707450.047.5305826
GTATCAA4657700.047.12539
TGGTATC4797200.046.42547
ATCAACG4397000.026.84816710-11
AGTACAT4516300.026.70079620-21
TACATGG4068600.026.30720322-23
CAACGCA4465400.026.29255912-13
AGAGTAC4659950.026.05168218-19
CATGGGG2148700.025.77047524-25
GCAGAGT4609100.025.6388716-17
ACGCAGA4600350.025.52086614-15
CAGAGTA4533550.024.07483518-19
CGCAGAG4430300.023.98614916-17
GTACATG4277900.023.8548922-23
Produced by FastQC (version 0.11.5)