FastQCFastQC Report
Tue 4 Jun 2019
HKCHVBGXB_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKCHVBGXB_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences354491733
Sequences flagged as poor quality0
Sequence length8
%GC89

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGG27912532878.73958741937714No Hit
CTAGACCT198568985.6015122925306695No Hit
GAGATGAC177340095.002657988642008No Hit
ACTTCATA172287654.860131674777307No Hit
TGCCGTGG114599753.232790480899592No Hit
GGGTGGGG5518950.15568628225245523No Hit
GGGGGGGT5293060.14931406030842476No Hit
GGTGGGGG3839810.10831874603969961No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)