FastQCFastQC Report
Tue 4 Jun 2019
HKCHVBGXB_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKCHVBGXB_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences354491733
Sequences flagged as poor quality0
Sequence length29
%GC86

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGG26662200975.21247582944339No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCGGAA223150.018.885321
GTAATCG291850.018.0665191
TCGGGAC172750.017.948371
CCGGACA125150.017.448161
CTTGGCG144000.017.07376710
ATGGGAG135400.016.9345231
CCTAAGA217950.016.8601861
TGAGGAG324850.016.854551
GAGAGAA193000.016.821755
TATCGCC191500.016.773781
CGGGACG99400.016.7301622
AGAGAAG200500.016.691636
GCACGCA160050.016.648833
ATAGCGA87750.016.5005636
TAGCACA262150.016.4487921
GTGCAGC242000.016.4349481
TCCGGGA205150.016.4147971
GGTGGAA142600.016.3463787
AAGGACC104300.016.2308672
GACACGC167800.016.1809621
Produced by FastQC (version 0.11.5)