FastQCFastQC Report
Wed 5 Apr 2017
HKCHCBCXY_l02n02_ngn_9_3.35200000086484.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKCHCBCXY_l02n02_ngn_9_3.35200000086484.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences87550032
Sequences flagged as poor quality0
Sequence length51
%GC30

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACCGCG149700.019.4486167
AGTCTGA224550.018.96811733
GCGGTAG156400.017.91081211
GTCTGAA238300.017.87356834
CGCGGTA167050.016.8361310
TAACCGC177400.016.5766776
GGACGAC155250.016.4200527
CGTCCAG131550.016.31684910
CGGTAGC172050.016.2816112
CTGGACG185350.016.2783725
ACCGCGG183550.015.8496858
CCTGGAC191400.015.7755714
TAGATAG176900.015.7586225
ACTGATA180850.015.5513478
GTCGTCC118300.015.5386698
TGGACGA199700.015.3903376
TCGTCCA147500.015.3455949
CCGTTTG112900.015.2655549
TATACTG259300.015.1414585
ATACTGA200400.015.0783496
Produced by FastQC (version 0.11.2)