FastQCFastQC Report
Wed 5 Apr 2017
HKCHCBCXY_l02n02_mesp_dnfgfr_10hpf_22_3_17.35200000086441.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKCHCBCXY_l02n02_mesp_dnfgfr_10hpf_22_3_17.35200000086441.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences99949849
Sequences flagged as poor quality0
Sequence length51
%GC30

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACCGCG195550.020.457467
GCGGTAG205750.018.76552411
CGCGGTA217100.017.90865710
TAACCGC226400.017.729486
CGGTAGC226600.016.97929612
ACCGCGG236300.016.7772228
AGTCTGA254300.016.62517733
GGACGAC189900.016.5401047
CTGGACG216500.016.2226495
CCGCGGT243050.016.2002189
CGTCCAG147550.015.90465410
GGCACGT240250.015.82753620
GTCGTCC137800.015.4134768
GTCTGAA274300.015.371881534
TGGACGA234650.015.1979956
CCTGGAC231950.015.1905414
GCACGTT259750.014.82989921
TGGCACG261550.014.58159319
TCTCGGT61300.014.57140241
TACCTGG245600.014.5567952
Produced by FastQC (version 0.11.2)