FastQCFastQC Report
Wed 5 Apr 2017
HKCHCBCXY_l02n02_lacz_8_3.3520000008646a.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKCHCBCXY_l02n02_lacz_8_3.3520000008646a.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25958817
Sequences flagged as poor quality0
Sequence length51
%GC36

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACCGCG38850.012.5095027
TAGATAG51300.012.2805085
CTGGACG44450.011.8445835
GGACGAC44150.011.7212657
AGTCTGA54600.011.6621533
CGTCCAG34000.011.44845910
TATACTG97000.011.4353875
ACCGCGG40150.011.1518198
GCGGTAG37800.011.01194311
CCTGGAC49050.010.9172584
GTATAAG127300.010.8520411
TAGACCG41000.010.64617355
GCTACTC38450.010.4744363
CCTATTC69000.010.3695019
GTCGTCC37900.010.3297118
CTGACGC43250.010.24827545
TGGACGA55050.010.1360996
CCGCGGT43450.010.0977529
TCTGACG62600.010.063621545
GTCTGAA63900.09.99999634
Produced by FastQC (version 0.11.2)