FastQCFastQC Report
Wed 5 Apr 2017
HKCHCBCXY_l02n02_gfp_6000_22_3.35200000086427.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKCHCBCXY_l02n02_gfp_6000_22_3.35200000086427.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3458535
Sequences flagged as poor quality0
Sequence length51
%GC32

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTCTGA7800.017.01924133
AACCGCG5400.016.249547
GTCTGAA8700.015.258409534
CGTCCAG4700.014.8402210
GGACGAC6250.014.75958257
TAGTACC3800.014.2101254
GTCGTCC5050.013.8114918
GCGGTAG6100.013.64735311
GCTGACC3151.8189894E-1213.57163324
CTGGACG6850.013.4667725
GCTCTCG5950.013.234927
GACCCTT3251.8189894E-1213.15442527
ACTGATA8400.013.1246298
CCTGGAC7200.013.1246294
TAGGCCC1200.001657932813.1246284
GTCCAGG5500.013.09072811
TGCTCTC6050.013.0163510
TATCCCG2602.6702764E-912.9804035
CGGTAGC6450.012.90679812
CCTATTC10700.012.8269269
Produced by FastQC (version 0.11.2)