FastQCFastQC Report
Wed 5 Apr 2017
HKCHCBCXY_l02n02_foxf_23_3.352000000864ae.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKCHCBCXY_l02n02_foxf_23_3.352000000864ae.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7182387
Sequences flagged as poor quality0
Sequence length51
%GC34

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGACGAC15050.015.2489437
AACCGCG12150.014.9997777
GCGGTAG10900.014.44962911
CTGGACG15950.014.24743755
CGCGGTA13900.013.75878910
CGGTAGC11700.013.65392812
CCTGGAC17250.013.0432844
TACCTGG18350.012.9970822
TAGATAG8500.012.9703955
TGGACGA17900.012.9467356
ACCGCGG13250.012.9054688
CCGCGGT13250.012.3960429
TATGGTC14400.012.34373944
GTCGTCC11750.012.2551378
TAACCGC15200.012.1379786
AGTCTGA16150.012.12081633
GGCACGT13000.011.94246320
ACCTGGA19250.011.8050183
AGTCCTC10300.011.57765935
CGTCCAG11500.011.54330710
Produced by FastQC (version 0.11.2)