FastQCFastQC Report
Wed 5 Apr 2017
HKCHCBCXY_l02n02_foxf_10_3.3520000008640d.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKCHCBCXY_l02n02_foxf_10_3.3520000008640d.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1775242
Sequences flagged as poor quality0
Sequence length51
%GC31

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGGTA2700.017.49992410
AACCGCG2550.016.7646337
AGTCTGA5300.015.70836333
CTAAGAC1754.8683432E-815.4285049
AGCTAAG2505.456968E-1215.2999337
GGCACGT2701.8189894E-1215.0007820
CGTCCAG2253.0559022E-1014.99993410
GTCTTAG3050.014.7523721
ACCGCGG2900.014.7413158
GTCTGAA5650.014.73527934
GCGGTAG2601.0913936E-1114.7110611
CGGAATT2001.5739715E-814.6257631
GTGTGCA1402.2314101E-514.4642239
AAAGTCT6000.014.25074131
CAACCGT1901.3337194E-714.2100646
TCGTCCA3250.013.8460929
GCGGAAT2154.0719897E-813.60535830
CCCTCAA2401.2119926E-813.12568230
GGACGAC2754.4019544E-1013.0908527
AAGTCTG6550.013.05411332
Produced by FastQC (version 0.11.2)