FastQCFastQC Report
Wed 5 Apr 2017
HKCHCBCXY_l02n01_ngn_9_3.35100000086487.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKCHCBCXY_l02n01_ngn_9_3.35100000086487.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences87550032
Sequences flagged as poor quality0
Sequence length51
%GC30

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACCGCG154300.021.2605047
AGTCTGA226800.019.65261733
GCGGTAG160700.019.22348611
GTCTGAA242850.018.31671134
CGCGGTA172300.018.2296210
TAACCGC183750.017.8655196
CGGTAGC175000.017.61406312
GGACGAC165400.017.0178137
CGTCCAG136650.016.90979410
ACCGCGG189850.016.8764618
CTGGACG196500.016.63765
CCTGGAC198950.016.5231724
TAGATAG172600.016.438495
GGCACGT189250.016.3116820
GTCGTCC122350.016.219848
CCGCGGT195950.016.1557129
TGGACGA207700.016.0004316
CCGTTTG117850.015.9225899
TACCTGG209000.015.89090252
TATACTG257250.015.8485845
Produced by FastQC (version 0.11.2)