FastQCFastQC Report
Wed 5 Apr 2017
HKCHCBCXY_l02n01_mesp_dnfgfr_10hpf_22_3_17.35100000086444.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKCHCBCXY_l02n01_mesp_dnfgfr_10hpf_22_3_17.35100000086444.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences99949849
Sequences flagged as poor quality0
Sequence length51
%GC30

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACCGCG201700.020.257747
GCGGTAG209000.018.76416811
CGCGGTA221200.018.10560810
AGTCTGA256700.018.047233
TAACCGC229600.017.6591246
GGACGAC205600.017.5643867
CTGGACG228350.017.4798975
CGGTAGC228400.017.19988412
ACCGCGG242350.016.7205968
GTCTGAA280000.016.52934334
TGGACGA247800.016.3167276
CCTGGAC246400.016.2815634
CCGCGGT250200.016.0969499
CGTCCAG144800.015.973610
GGCACGT247600.015.86627220
TACCTGG257150.015.8378942
GTCGTCC133050.015.8116718
CCGAGCC57900.015.69943720
TGGCACG264750.014.96592519
GCACGTT263300.014.89456521
Produced by FastQC (version 0.11.2)