FastQCFastQC Report
Wed 5 Apr 2017
HKCHCBCXY_l02n01_lacz_8_3.3510000008646d.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKCHCBCXY_l02n01_lacz_8_3.3510000008646d.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25958817
Sequences flagged as poor quality0
Sequence length51
%GC36

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCCGAG26350.016.8215145
AACCGCG40550.012.9284397
AGTCTGA55050.012.75197633
CTGGACG45050.012.2364345
ACCGCGG38300.011.8667788
GGACGAC45850.011.8265697
GCTACTC37700.011.7574823
CGTCCAG34050.011.56381510
TAGATAG49550.011.4430145
GCGGTAG35450.011.42448111
CCTGGAC48550.011.2152484
CCTATTC68300.010.87107859
TCGTCCA45150.010.86370859
CTACTCT68850.010.8496784
TATACTG96900.010.7972395
GTATAAG130050.010.6763291
GTCGTCC36150.010.5808578
TAGACCG43000.010.5697935
GCCTAAT65300.010.5107521
GTCTTAG41800.010.4979731
Produced by FastQC (version 0.11.2)