FastQCFastQC Report
Wed 5 Apr 2017
HKCHCBCXY_l02n01_gfp_6000_22_3.3510000008642a.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKCHCBCXY_l02n01_gfp_6000_22_3.3510000008642a.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3458535
Sequences flagged as poor quality0
Sequence length51
%GC32

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGGTAG6550.018.20561611
AACCGCG6950.018.1290077
AGTCTGA7450.017.21479833
TAACCGC7650.016.7644966
CGCGGTA7550.016.09228110
GTCTGAA8300.015.99400734
CCGCGGT7550.015.4962719
CGGTAGC7550.015.49627112
TCGGATC6150.015.36543813
ACCGCGG7900.015.0945298
ATACTGA8200.015.0912736
TAGATAG6950.014.8918995
GGACGAC6650.014.8868167
AATAGGG8700.014.7409827
CTGGACG6950.014.5681625
CGTAGTA6800.014.558640526
CATCACG7150.014.4765992
TCACGTC7050.014.3615234
TGGCACG8200.014.26790719
GGCACGT8250.014.1816420
Produced by FastQC (version 0.11.2)