FastQCFastQC Report
Wed 5 Apr 2017
HKCHCBCXY_l02n01_foxf_23_3.351000000864a1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKCHCBCXY_l02n01_foxf_23_3.351000000864a1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7182387
Sequences flagged as poor quality0
Sequence length51
%GC34

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACCGCG11350.017.246817
GCGGTAG11600.016.0989111
CTGGACG14950.015.9536175
GGACGAC15500.015.6775247
CGGTAGC12050.015.497704512
GGCACGT12400.015.24171920
GTCGTCC13200.015.170458
CGTCCAG13150.015.0568210
CCTGGAC15950.014.953394
TACCTGG16400.014.8183062
AGTCTGA14850.014.24222233
ACCGCGG14300.014.1608358
CGCGGTA14300.013.84595810
GCACGTT14750.013.27099821
TGGCACG14600.013.25323619
ACCTGGA18550.013.2218483
TGGACGA18550.013.2213886
CCGCGGT14700.013.1630779
GTCCAGG15500.013.0643311
GTCTGAA16600.012.87632334
Produced by FastQC (version 0.11.2)