FastQCFastQC Report
Wed 5 Apr 2017
HKCHCBCXY_l02n01_foxf_10_3.35100000086400.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKCHCBCXY_l02n01_foxf_10_3.35100000086400.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1775242
Sequences flagged as poor quality0
Sequence length51
%GC31

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACCGCG3350.017.4626227
ACCGCGG3750.017.3999358
GGCACGT3600.016.24993920
TAGCTGG3600.016.24993915
GCGGTAG3750.016.1999411
AGTCTGA3650.016.02733833
GTCGTCC2302.5465852E-1115.6521158
ACTGATA2750.015.5453978
CGTCCAG2202.1827873E-1015.34085210
CAACCGT2353.6379788E-1115.3190916
GTCCAGG2253.0559022E-1014.99994311
CCGCGGT3950.014.8100719
GGACGAC3650.014.7944657
GTCTTAG2900.014.7429851
CGGTAGC4250.014.29406412
GTCTGAA4100.014.26823934
CGCGGTA4100.014.26823910
GCACGTT4150.014.096333521
CGTTAAT4150.014.096333524
TGGCACG4150.014.096333519
Produced by FastQC (version 0.11.2)