FastQCFastQC Report
Wed 5 Apr 2017
HKCHCBCXY_l01n02_ngn_22_3.35200000086477.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKCHCBCXY_l01n02_ngn_22_3.35200000086477.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences40048545
Sequences flagged as poor quality0
Sequence length51
%GC36

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGGTGG48250.012.26459843
AACCGCG66300.011.7418147
TCTCGGT53300.011.3972941
CGTCCAG46950.011.30970410
GCTGCCG60350.011.29702323
CTGCCGA56500.011.27034524
CTGGACG60900.011.0094785
CCGACGA56550.010.94195627
ACCGCGG63800.010.6149638
GCGGTAG57500.010.44766611
GGACGAC61450.010.2886197
CCTGGAC66050.010.2873114
GTCTTAG56300.010.2713551
CCGCGGT66250.010.1884869
TATACTG158000.010.1816165
CTCGGTG59350.010.08451942
GTCGTCC50900.09.9457458
CGCTGCC65100.09.885159522
TAGATAG71500.09.6290445
AGTCTGA74700.09.60832333
Produced by FastQC (version 0.11.2)