FastQCFastQC Report
Wed 5 Apr 2017
HKCHCBCXY_l01n02_ngn1_23_3.3520000008641a.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKCHCBCXY_l01n02_ngn1_23_3.3520000008641a.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences50929235
Sequences flagged as poor quality0
Sequence length51
%GC35

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACCGCG90950.014.1999457
GCGGTAG81950.013.64543811
CGTCCAG76100.013.30478710
AGTCTGA105050.012.8080533
GGACGAC91100.012.6452987
ACCGCGG96900.012.4688968
GTCGTCC79150.012.3940458
CGGTAGC91250.012.20543312
CTGGACG95150.011.6575445
CCTGGAC96600.011.5291424
GTCCAGG85050.011.48141611
CCGCGGT100650.011.4678819
GTCTGAA119850.011.43289834
TCGGTGG54850.011.28101243
TCGTCCA100600.011.20519359
GGCACGT98800.011.15897520
GTCTTAG83750.010.8813261
TAGCTGG102550.010.86056915
CGCGGTA111000.010.84453310
CCGACGA58950.010.83999427
Produced by FastQC (version 0.11.2)