FastQCFastQC Report
Wed 5 Apr 2017
HKCHCBCXY_l01n02_mesp_dnfgfr_10hpf_23_3_17.35200000086491.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKCHCBCXY_l01n02_mesp_dnfgfr_10hpf_23_3_17.35200000086491.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5264685
Sequences flagged as poor quality0
Sequence length51
%GC34

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTCTGA10500.014.35693133
AACCGCG8250.014.1814747
GTCCAGG7850.014.04437811
GCGGTAG7500.013.79979611
CGTCCAG7600.013.61809110
GTCGTCC7750.013.3545158
GTCTGAA12000.012.74981134
CGGTAGC8300.012.46969412
GGACGAC9600.011.7184657
GCCTAGC1802.3456044E-411.2507951
CTGGACG9500.011.1313095
TAGCTGG8950.011.06139415
TCGTCCA9600.011.0153579
GGCACGT9200.011.00548120
ACCGCGG9850.010.9642018
ACTGGGC3103.6188794E-810.8868338
AGTCCTC6250.010.79973735
GTATAAG23450.010.65107251
CCGTCCG1700.001839699510.5879789
TCCTTAG11350.010.50635210
Produced by FastQC (version 0.11.2)