FastQCFastQC Report
Wed 5 Apr 2017
HKCHCBCXY_l01n02_lacz1_22_3.3520000008645e.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKCHCBCXY_l01n02_lacz1_22_3.3520000008645e.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17814988
Sequences flagged as poor quality0
Sequence length51
%GC34

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACCGCG31800.015.2827577
GCGGTAG31000.014.29830311
CGGTAGC31850.013.42221412
AGTCTGA39300.013.33950533
CTGGACG32500.013.1534745
ACCGCGG36450.013.1479238
CGTCCAG26050.013.128448510
CGCGGTA34400.012.8196210
GGACGAC31450.012.5196597
GTCGTCC25750.012.4949328
GGCACGT35800.012.12995320
TAACCGC40450.011.9032486
TCGTCCA32600.011.80201159
CCTGGAC35750.011.7688984
GTCCAGG29550.011.72582111
GTCTGAA45050.011.68681534
AGTCCTC22100.011.50432935
CCGCGGT38350.011.4992169
GTATAAG80900.011.3479771
TGGACGA38800.011.1916936
Produced by FastQC (version 0.11.2)