FastQCFastQC Report
Wed 5 Apr 2017
HKCHCBCXY_l01n02_gfp_5000_27_2.35200000086434.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKCHCBCXY_l01n02_gfp_5000_27_2.35200000086434.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10637641
Sequences flagged as poor quality0
Sequence length51
%GC33

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGGACG21950.015.2728655
CCTGGAC22600.014.9331554
AACCGCG17550.014.8715337
GGACGAC19100.014.6070747
TGGACGA24650.014.1476926
TACCTGG24500.014.14234352
GTCCAGG16450.013.95112311
CGTCCAG16150.013.79232710
ACCGCGG19750.013.55672558
AGTCTGA20750.013.44554633
ACCTGGA26200.013.1387743
GTCGTCC14950.013.0934168
GTACCTG26750.013.0382591
ATCCGTG14500.012.87914416
GCGGTAG18350.012.874433511
TCGTCCA20050.012.5683589
TCGGTGG8000.012.3755443
CAGTTAG16950.012.0804561
GTCTGAA25000.012.05978934
CCAAGAT29600.011.85862543
Produced by FastQC (version 0.11.2)