FastQCFastQC Report
Wed 5 Apr 2017
HKCHCBCXY_l01n02_foxf_22_2.352000000863ff.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKCHCBCXY_l01n02_foxf_22_2.352000000863ff.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences40413820
Sequences flagged as poor quality0
Sequence length51
%GC35

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACCGCG68700.015.1962837
AGTCTGA90400.014.51047633
CTGGACG72900.014.5057455
GGACGAC70700.014.352697
CGTCCAG51200.014.06234610
GCGGTAG65200.013.94158211
CCTGGAC78800.013.4196554
ACCGCGG71350.013.1497738
GTCTGAA100050.012.75104834
GTCGTCC56650.012.3519818
CGGTAGC72750.012.09272612
CCGCGGT76400.011.9566759
TGGACGA91150.011.8976196
GGCACGT76650.011.74178720
CGCGGTA80750.011.61906810
GTATAAG201550.011.5546791
GTCCAGG59750.011.48524511
TACCTGG93950.011.4471132
TCGTCCA72900.011.1727179
TATACTG141450.011.0866295
Produced by FastQC (version 0.11.2)