FastQCFastQC Report
Wed 5 Apr 2017
HKCHCBCXY_l01n01_ngn_22_3.3510000008647a.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKCHCBCXY_l01n01_ngn_22_3.3510000008647a.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences40048545
Sequences flagged as poor quality0
Sequence length51
%GC36

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGAGCC50150.013.01026720
TCCGAGC51400.012.82518519
CTCCGAG52550.012.20200718
GCCCACG52850.011.96235424
CCACGAG56150.011.57977626
AGCCCAC58100.011.15248723
AACCGCG67950.011.1250497
ACCGCGG63600.010.9662268
CTGGACG61450.010.84250455
CCCACGA58950.010.72443925
CGTCCAG44950.010.31074710
CGAGACA64650.010.26610229
GCGGTAG56600.010.13622811
ACGAGAC68450.010.05773228
TATACTG159650.010.0526475
GAGCCCA67350.010.02169422
CGAGCCC65600.09.87751721
GTCTTAG57700.09.8703411
GGACGAC61150.09.8603077
CCTGGAC69200.09.6607344
Produced by FastQC (version 0.11.2)