FastQCFastQC Report
Wed 5 Apr 2017
HKCHCBCXY_l01n01_ngn1_23_3.3510000008641d.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKCHCBCXY_l01n01_ngn1_23_3.3510000008641d.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences50929235
Sequences flagged as poor quality0
Sequence length51
%GC35

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACCGCG86350.014.9034047
CTCCGAG41700.014.51356318
GGACGAC93300.014.3719757
GCGGTAG79750.014.13375511
TCCGAGC44750.014.02713419
CTGGACG93850.013.9350665
CCGAGCC45500.013.69702220
CGAGACG46400.013.38263529
GACGCTA46700.013.29666532
ACCGCGG92750.013.2685648
AGTCTGA104800.012.90219933
CGGTAGC87500.012.83048312
CCTGGAC101250.012.7832134
GCTACGC49800.012.64967135
CGCTACG49700.012.40351334
CCGCGGT97200.012.1750299
CGTCCAG72750.012.15374410
GCCCACG53450.012.03850824
TGGACGA115650.012.02846056
GTCGTCC76200.011.8692058
Produced by FastQC (version 0.11.2)