FastQCFastQC Report
Wed 5 Apr 2017
HKCHCBCXY_l01n01_mesp_dnfgfr_10hpf_23_3_17.35100000086494.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKCHCBCXY_l01n01_mesp_dnfgfr_10hpf_23_3_17.35100000086494.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5264685
Sequences flagged as poor quality0
Sequence length51
%GC34

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACCGCG9500.016.8145627
GCGGTAG9450.015.95121611
ACCGCGG9950.015.6018768
CCGCGGT10500.013.71328459
CGGTAGC10900.013.62285912
AGTCTGA9100.013.59790833
CGCGGTA11250.013.39902110
GGCACGT10850.013.06368520
GGACGAC8800.012.7831587
GTCTGAA10300.011.79525534
CGGGACT5500.011.4537110
GCACGTT12400.011.43072521
GTCGTCC8550.011.3149628
TAACCGC12950.011.2982996
TAGCTGG12550.011.29410415
TATACCG13600.011.2548445
TGGCACG13650.011.20807819
CTGGACG9650.011.1965285
CGTCCAG8150.011.04213810
CAGTTAG7650.010.887971
Produced by FastQC (version 0.11.2)